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The cells were washed with serum-free medium three times to remove excess DCFH-DA and immediately analysed using the FACSCanto II flow cytometer
Aortic tissue LOXL2 activity assay Aortas from young ( 18 months old) WT and LOXL2+/ littermates were extracted and cleaned free of connective tissue in Krebs-HEPES buffer (NaCl 135 mM, KCl 5 mM, MgSO 4 1 mM, K 2 HPO 4 0.4 mM, Glucose 5.5 mM, HEPES 20 mM, adjust the pH to 7.4, supplement with 1 mM CaCl 2 ) containing 0.25 g/mL fungizone (Gibco)
doi: 10.1677/joe.0.1300267 56 KimS-JNianCKarunakaranSCleeSMIsalesCMMcIntoshCHS
There are still significant knowledge gaps regarding the long-term GI safety of GLP-1-based therapies and the optimal management of persistent or delayed-onset symptoms
The degree of nausea often correlates with the potency and dose of the medication
You can also easily find these at most drugstores, so maybe you can swing by and chat with your local pharmacist
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